Background
"IgG is a monomeric immunoglobulin. It is the most abundant immunoglobulin and is found in the blood and extracellular fluid. There are four subclasses of IgG: IgG-1, IgG-2, IgG-3 and IgG-4. IgG is composed of two heavy (γ) chains and two light (κ or λ) chains. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Each IgG Fab fragment has two antigen binding sites. IgG molecules are involved in secondary immune response. They bind to several different kinds of pathogens, such as viruses, bacteria and fungi, and protect the body by complement activation (the classic pathway), opsonization for phagocytosis, and neutralization of toxins. In addition, IgG is the only isotype that can pass through the placenta, thereby providing protection to the fetus in the first weeks of life, before immune system of the fetus has developed."
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|