AKT1/PKBa(pT308)
v-akt murine thymoma viral oncogene homolog 1; AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
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Antigen Preparation
A synthetic peptide containing the phospho-Thr308 motif (GATMKTFCG) of human AKT1.
Background
"Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (α), Akt2 (β) and Akt3 (γ)]; Akt is activated by insulin and growth and survival factors. Akt1 is phosphorylated at Thr308 and Ser473. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
"The motif corresponding to the major autophosphorylation site of the human AKT1 kinase protein. This antibody does not cross react to the non-phosphorylated AKT1 or with other unrelated phosphorylated serine. Thr308 corresponds to GATMK in human, rat, mouse, chicken and canis of AKT/PKB proteins"
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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