Antigen Preparation
"A synthetic peptide corresponding to the C-terminus of human FOXA2 protein. This sequence is identical to rat, mouse and human origins."
Background
"The forkhead box gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA1 plays an important role in breast cancer. The FOXA subfamily is composed of the transcription factors FOXA1, A2, A3. They were discovered as the regulators of hepatic genes. Regularly, the plasma insulin inhibits the forkhead transcription factor Foxa2. The cytoplasmic localization and inactivation of Foxa2 are observed in the chronic hyperinsulinaemia of insulin-resistant syndromes, thereby promoting lipid accumulation and insulin resistance in the liver. Pharmacological intervention to inhibit phosphorylation of Foxa2 may be an effective treatment for type 2 diabetes."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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