Antigen Preparation
"A synthetic peptide GVVSIFGVASR corresponding to the N-terminus of Fibroblast Growth Factor 4 protein. This sequence is identical to human, mouse, rat and bovine"
Background
"FGF4 is a member of fibroblast growth factor (FGF) family. The FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. FGF4and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway"
Applications/Suggested Working Dilutions
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|