Antigen Preparation
"A synthetic peptide derived from the c-term of human AEG1. This sequence is identical to human, mouse, rat and bovine. "
Background
"AEG1 (Astrocyte elevated gene 1), also called Metadherin (MTDH), or L LYsine-RIch CEACAM1 co-isolated (LYRIC), is 582 aa single transmembrane domain (TMD) protein, this protein is widely expressed and highly conserved between species. It is not a structural component for tight junctional (TJ) complexes, it is recruited during the maturation of the tight junction complex and co-localizes with tight junction proteins ZO-1 and occludin in polarized epithelial cells. This novel protein is also reported as an HIV-1 inducible gene, which is expressed ubiquitously with higher expression in tissues containing muscular actin and its expression is increased in astrocytes infected with HIV-1 or treated with gp120 or tumor necrosis factor (TNF)-alpha. AEG1 expression is elevated in subsets of breast carcinomas, malignant gliomas and melanomas. AEG1 may affect tumor progression in multiple cell lineages by augmenting expression of the transformed phenotype and/or by inducing glutamate excitotoxicity in malignant glioma. In these contexts, an HIV-1-inducible gene, AEG-1, may contribute to multiple brain abnormalities and tumor formation, by both common and distinct mechanisms."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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