AKT1 (paried T308)
v-akt murine thymoma viral oncogene homolog 1; AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
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Antigen Preparation
"A synthetic peptide KDGATMKTFCG of human AKT1. This sequence is identical to human, mice and rat"
Background
"Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (α), Akt2 (β) and Akt3 (γ)]; Akt is activated by insulin and growth and survival factors. Akt1 is phosphorylated at Thr308 and Ser473. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody recognize AKT1 protein without phosphorylation site at Threonine 308. It does not cross react to the phosphorylated AKT1 at Thr308. The antigen motif corresponds to GATMKTFCG in human, rat, mouse, chicken and canis of AKT/PKB proteins"
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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