c-erbB2 (pY1221/Y1222) (HER2)
c-erbB2/neu protein
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Antigen Preparation
A synthetic peptide containing the epitope FDNL-Y*-Y*-WDQ of c-erbB2 with dual phosphorylation sites Tyrosine 1221 and 1222.
Background
"Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
This antibody recognizes c-erbB2 with the phosphorylation sites tyrosine 1221/1222. It does not cross-react with non-phosphospecific peptide.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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