Antigen Preparation
"A synthetic peptide PFGGFKMSGNGRELGE corresponding to human ALDH1a1. It is identical among human, mice and rat."
Background
"ALDH1a1 belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. ALDH1A1 is also involved in retinol metabolism where retinol is converted to retinoic acid. Hematopoietic stem cell differentiation is suggested to be mediated through ALDH1A1 control of the retinoid signaling pathway."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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