Cell Division cycle 25c

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Antigen Preparation
A synthetic peptide surrounding of –-YRPSMPE-- of human CDC25C protein with a phosphorylation site Serine 216.
"CDC25c is a tyrosine phosphatase and belongs to the CDC25 phosphatase family. The three homologues of CDC25 (CDC25A/B/C Cdc25) tyrosine phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes CDK1 (CDC2). Cdc25C, a dual-specificity protein kinase controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. Cdc25C is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to Cdc25C, and Cdc25C/14-3-3 complexes are present throughout interphase but not during mitosis. C-TAK1 (for Cdc twenty-five C associated protein kinase) can phosphorylate Cdc25C on serine 216 in vitro. "
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
This antibody recognizes human CDC25C(pS216) protein with a phosphorylation site Serine 216.
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
0.01-0.1 µg/ml
2-5 µg/ml
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 601-640
Lot #: See the label
Size: 100ug/200ul
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC(P), WB
Reactivity: Hu
Price: $ 245.00
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