CDC25C(pS216)
Cell Division cycle 25c

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Product Preparation
N/A
Background
CDC25c is a tyrosine phosphatase and belongs to the CDC25 phosphatase family. The three homologues of CDC25 (CDC25A/B/C Cdc25) tyrosine phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes CDK1 (CDC2). Cdc25C, a dual-specificity protein kinase controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. Cdc25C is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to Cdc25C, and Cdc25C/14-3-3 complexes are present throughout interphase but not during mitosis. C-TAK1 (for Cdc twenty-five C associated protein kinase) can phosphorylate Cdc25C on serine 216 in vitro.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
N/A
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
995
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 601-640
Lot #: See the label
Size: 100 ug
Host: Rabbit
Clone:
Isotyping: Rabbit IgG
Applications: Elisa, WB, IHC
Reactivity: Hu
Price: $ 245.00
REFERENCES
Peng CY1, Graves PR, Ogg S, Thoma RS, Byrnes MJ 3rd, Wu Z, Stephenson MT, Piwnica-Worms H. C-TAK1 protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding. Cell Growth Differ. 1998 Mar;9(3):197-208.
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