Antigen Preparation
A recombinant protein of full length human AID.
Background
"AID, also known as Activation-induced cytidine deaminase, an RNA-editing deaminase, is a 24 kDa enzyme that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion (in chickens, pigs, etc.), and class-switch recombination of immunoglobulin genes. It creates mutations in DNA by deamination of cytosine base, which turns it into uracil which is recognized as a thymine. AID plays a critical role during B cell development. Protein is normally expressed in germinal center B cells, its forced expression in B cells at the wrong stage of differentiation, in non-B cells, and in Escherichia coli results in enhanced mutagenesis and mutational spectra mimicking somatic hypermutation (SHM). Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). Overexpression correlates with poor prognosis in chronic lymphocytic leukemia and lymphoma"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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