AID

Product Preparation
Background
AID, also known as Activation-induced cytidine deaminase, an RNA-editing deaminase, is a 24 kDa enzyme that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion (in chickens, pigs, etc.), and class-switch recombination of immunoglobulin genes. It creates mutations in DNA by deamination of cytosine base, which turns it into uracil which is recognized as a thymine. AID plays a critical role during B cell development. Protein is normally expressed in germinal center B cells, its forced expression in B cells at the wrong stage of differentiation, in non-B cells, and in Escherichia coli results in enhanced mutagenesis and mutational spectra mimicking somatic hypermutation (SHM). Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). Overexpression correlates with poor prognosis in chronic lymphocytic leukemia and lymphoma
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
FORMULATION
STORAGE
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 604-040
Lot #:
Size: 100 ug
Host: Mouse
Clone: ZY201
Isotyping: IgG1/k
Applications: WB, IHC-P, IF, IP
Reactivity: Hu
Price: $ 245.00
REFERENCES
Bransteitter R, et al.(2003) Proc. Natl. Sci USA 100 (7) Martin A et al. (2002) Nature 415 Martin A et al. (2002) Proc Natl Acad Sci USA. 2002;99 Yoshikawa K, et al. (2002) Science 296 Petersen-Mahrt S K et al. (2002) Nature 418 Harris R S et al.(2002) Mol Cell 10
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