Antigen Preparation
A recombinant protein of full length human 4E-BP1
Background
"4E-BP1 eukaryotic initiation factor 4E binding protein 1 is a member of a family of translation repressor proteins. It inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. In the unphosphorylated form, it suppresses the translation initiation of capped mRNAs, whereas in phosphorylated form, it increases translation initiation. Several kinases phosphorylate 4E-BP1 dependent or independent of mTOR, indicating that mTOR may not be the only kinase that phosphorylates 4E-BP1. It is a multi-functional protein, in addition to translation repression, under certain cellular conditions it may also play a role as a tumor promoter. The protein is involved in tumorigenesis and is overexpressed in various carcinomas."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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