Cell Division cycle 25b

PDF Image Download Product PDF PDF Image MSDS Download Product PDF
Antigen Preparation
A synthetic peptide surrounding of –LGSPV- of human CDC25B protein with a phosphorylation site Serine 42.
"Three homologues of CDC25 called CDC25A/B/C Cdc25 tyrosine phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes CDK1 (CDC2). Dephosphorylation of threonine 14 and tyrosine 15 of CDK1 by CDC25 phosphatases is a key step in the activation of the CDK1-cycline B protein kinase. Of the three mammalian Cdc25 isoforms, Cdc25A is phosphorylated by genotoxic stress-activated Chk1 or Chk2, which triggers its SCFbeta-TrCP-mediated degradation. CDC25B is degraded by the proteasome. CDK1-cyclin A phosphorylation targets CDC25B for degradation and this might be an important component of cell cycle regulation at the G2/M transition. "
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
This antibody recognizes phosphorylated Serine 42 of human CDC25B protein. It does not recognize the nonphosphorylated CDC25B.
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
0.01-0.1 µg/ml
2-5 µg/ml
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 601-610
Lot #: See the label
Size: 100ug/200ul
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC(P), WB
Reactivity: Hu
Price: $ 245.00
©2011-2025 All rights reserved.