Abbomax

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Actin-beta

ACTB

Antigen Preparation

Background

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three α-actin (skeletal, cardiac, and smooth muscle), one β-actin (β non-muscle), and two γ-actins (γ-smooth muscle and γ-non-muscle). Antibody to β-actin provides a specific and useful tool in studying the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This beta-actin is a major constituent of the contractile apparatus and one of the two non-muscle cytoskeletal actins.

PURIFICATION The Rabbit IgG is purified by Epitope Affinity Purification	SPECIFICITY
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.	STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

6382

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

5-10 µg/ml

Order Info

Catalog #:	500--4554
Lot #:
Size:	100 ug
Host:	Rabbit
Clone:
Isotyping:	Rabbit IgG
Applications:	IHC(P)
Reactivity:	Hu
Price:	$ 245.00

REFERENCES

Harris,D.E., et al; Nucleotide sequences of the rabbit alpha-smooth-muscle and beta-non-muscle actin mRNAs. Gene 112 (2), 265-266 (1992)