AKT1/PKBa
v-akt murine thymoma viral oncogene homolog 1; AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
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Antigen Preparation
A synthetic peptide corresponding to the close to C-term of human AKT1 prote
Background
"Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (α), Akt2 (β) and Akt3 (γ)]; Akt2 and Akt3 have 81% and 83% homology in amino acid sequences with Akt1, respectively. Numerous studies have revealed that the expression pattern of Akt1 and Akt2 is ubiquitous, whereas Akt3 is expressed predominantly in brain, heart, and kidney. Akt is activated by insulin and growth and survival factors .Akt has been referred to as an oncogene because it has increased activity in a number of tumors."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody only recognizes ~60 kDa of human AKT1, not AKT1 or AKT2. It also reacts with mouse and rat AKT3 protein. The other species are not tested. "
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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