BCAR3 (pT130)
breast cancer anti-estrogen resistance 3
Download Product PDF
MSDS Download Product PDF
Antigen Preparation
"A synthetic peptide surrounding of RHIMDR-T*-PEKLK of human BCAR3 protein with a single phosphorylation site at Thr 130. This sequence is identical to human, rat, mouse, canis and bovine."
Background
"Breast cancer anti-estrogen resistance 3 (BCAR3) is a also known as AND-34/BCAR3/NSP2 (BCAR3). Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. BCAR3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48"
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
|
SPECIFICITY
This antibody recognizes phosphorylated Threonin 130 of human BCAR3 protein. It does not recognize the nonphosphorylated BCAR3.
|
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
|
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
|
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|