Antigen Preparation
"A synthetic peptide surrounding to the epitope -DGYVK-- with phosphorylation sites at Tyr694 of STAT5 protein from human, mouse and rat origins."
Background
"STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. STAT5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a co-activator for both the a- and b-isoforms of STAT5."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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