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14-3-3 beta

YWHAB; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide

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Antigen Preparation

A synthetic peptide corresponding to N-term of human 14.3.3 beta protein.

Background

"The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14.3.3 beta is highly expressed in brain tissue. It is critical to cell transformation and mitotic signaling."

PURIFICATION The Rabbit IgG is purified by Epitope Affinity Purification	SPECIFICITY "This antibody recognizes ~28 kDa of human 14.3.3 beta. It does not react to the other isoforms. This antibody reacts with human, rat and mouse. The other species are not tested."
FORMULATION This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer	STORAGE The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

7529

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

Not tested

Order Info

Catalog #:	500-2924
Lot #:	See the label
Size:	100ug/200ul
Host:	Rabbit
Clone:
Isotyping:	Rabbit IgG
Applications:	IHC(P), WB, IP
Reactivity:	Hu, Ms, Rt
Price:	$ 189.00

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