STAT4(pY693)
Signal transducer and activator of transcription 4

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Product Preparation
A synthetic peptide surrounding to the epitope -KGYVP-- with phosphorylation sites at Tyr693 of STAT4 protein from human, mouse, rat, chicken dog and bovine origins.
Background
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. Activation of STAT4 induces dimerization, nuclear translocation and DNA binding. STAT4 protein expression is restricted to the thymus, spleen and testis. Both tyrosine and serine residues on STAT4 become phosphorylated in response to IL-12 or IFN-a although serine phosphorylation is not required for DNA binding. STAT4 is activated in T-cells in response to the cytokine interleukin-12 (IL-12), and in NK cells by IL-2. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes STAT4 (pY693) with a phosphorylated sites of Tyr693. It does not cross-react with non-phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
6775
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 500-12064
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC, ELISA, IP
Reactivity: Hu, Ms
Price: $ 255.00
REFERENCES
Takuya Miyagi, et al. High basal STAT4 balanced by STAT1 induction to control type 1 interferon effects in natural killer cells. J Exp Med. 2007 October 1; 204(10): 2383–2396.
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