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CD4

CD4 antigen; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3.

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Antigen Preparation

Human T cells expressed CD4

Background

"CD4 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. This anti-CD3 antibody is commonly used as a phenotypic marker for human T cells."

PURIFICATION The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping	SPECIFICITY This antibody recognizes human CD4 protein. The other species was not tested.
FORMULATION This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer	STORAGE The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

920

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

0.5-5 µg/106 cells

Order Info

Catalog #:	603-040
Lot #:	See the label
Size:	100ug/200ul
Host:	Mouse
Clone:	S3.5
Isotyping:	IgG2a
Applications:	IHC(P), FC
Reactivity:	Hu
Price:	$ 245.00

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