P53(pS15)
Tumor protein p53; TP53; tumor suppressor phosphop

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Product Preparation
A synthetic peptide corresponding to the epitope –PLSQE- at a phosphorylation site at serine 15 of human p53 protein
Background
P53, also known as protein 53, or tumor suppressor protein 53, plays important roles in cellular response to DNA damage and other genomic aberration. Mutations in the p53 tumor suppressor gene giving rise to mutant p53 proteins are among the most common genetic alterations associated with tumor cells. P53 protein is phosphrylated at multiple sites in vivo and in vitro in response to different stimuli. UV caused DNA damage can induce Ser9, Ser15, Ser20 phosphorylation while ATM, ATR, DNA-PK at Ser15 and Ser37.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~53 kDa of humanP53 protein. It does not recognize non-phosphorylated peptides. This antibody also reacts with mouse and rat. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
7157
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-550
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, ELISA
Reactivity: Hu, Ms, Rt
Price: $ 220.00
REFERENCES
Sylvie Labrecque, et al. Analysis of the Anti-p53 Antibody Response in Cancer Patients (1993) Cancer Research 53, 3468-3471
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