PAK4(pS474)
p21 protein (Cdc42/Rac)-activated kinase 4

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Product Preparation
A synthetic peptide surrounding the epitope RKSLV-with a phosphorylation at Serine 474 of human PAK4. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species.
Background
p21-activated protein kinase (PAK) serine/threonine kinases are important effectors of Rho family GTPases and have been implicated in the regulation of cell morphology and motility, as well as in cell transformation. The phosphorylation of serine 474 as the likely autophosphorylation site in the kinase domain of PAK4 occurs in vivo. PAK4 is frequently over-expressed in human tumor cell lines of various tissue origins, indicates that the phosphorylation of PAK4 is required in oncogenic transformation and Ras-driven, anchorage-independent growth.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes the phosphorylated peptide only. It recognizes PAK4 protein at a phosphorylation site of Serine 474(~68kDa), or serine 602 of PAK5 (~90kDa) or Serine 560 of PAK6 (~80 kDa). It does not cross react to other PAK1, PAK2 or PAK3. This antibody reacts with human, rat and mouse. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
10298
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-030
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, IP, ELISA
Reactivity: Hu, Ms, Rt
Price: $ 365.00
REFERENCES
Marinella G. Callow et al. Requirement for PAK4 in the Anchorage-independent Growth of Human Cancer Cell Lines. (2002) J. Biol. Chem. 277 (1), 550–558.
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