Bim
BCL2-like 11

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Product Preparation
A synthetic peptide derived from N-terminus of human BIM.
Background
Bim (bcl-2-interacting mediator of cell death, BCL-2-related Ovarian Death Gene) is a proapoptotic member of the Bcl-2 family that shares the homology domain3 (BH3) with this family. Bim exists in three isoforms: BimEL, BimL, and BimS. As the BCL-2 family members reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria, they play a pivotal role in deciding whether a cell will live or die. In healthy cells, BIM molecules are localized to the microtubule-associated dynein motor complex through association with the LC8 dynein light chain. Following a death signal, LC8 and BIM dissociate from the motor complex and together translocate to the mitochondria. At the mitochondria, BIM is thought to interact with BCL-2 to antagonize its antiapoptotic activity.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes BIM protein. It cross reacts to human, rat and mouse. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
N/A
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-4364
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC(P), WB
Reactivity: Hu, Ms, Rt
Price: $ 299.00
REFERENCES
1. Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science. 1998; 281(5381):1322-1326.
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