Antigen Preparation
A synthetic peptide corresponding to the epitope –TTSSDS-at a phosphorylation site Ser503 of human c-Rel protein. This sequence is identical within human and mouse.
Background
"The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA, RELB, NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor. C-Rel protooncogene can be formed immune complexes with othe proteins such as p105, p50 NFKB under phosphorylation status"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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