Antigen Preparation
"A synthetic peptide derived from internal sequence (-TS*GS*GS*G-) with a phosphorylation sites at Serine 187, 189 and 191 of mouse TGFbeta receptor type-1. This sequence is also identical within human, rat, mouse, chicken and other sp"
Background
"Transforming growth factor β isoforms (TGF-β1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal through a highly restricted subset of receptors known as TGF-β type I receptor (TβR-I) and TGF-β type II receptor (TβR-II). Expression of T beta R-I is more ubiquitous than that of T beta R-II. There was no staining of T beta R-II in the central nervous system, while intense T beta R-I staining was found specifically in nervous tissues. Expression of T beta R-I and T beta R-II was mostly coincident with that of their ligands, suggesting that TGF-beta s act as multiple mediators during organogenesis. TGF-β binds with high affinity to its receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, to form a dynamic dimer with phosphorylation in the cytoplasmic Ser/Thr residuals which cause its kinase activity and association with another TGF beta-binding protein, the type I receptor, to signal growth inhibition and early gene responses. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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