Antigen Preparation
"A synthetic peptide corresponding to --EAS T* PES-- of c-Fos protein at the phosphorylation site at Thr 232. This sequence is identical in human, mouse, rat."
Background
"The c-Fos proto-oncogene, an 55 kDa nuclear phosphor-protein, belongs to a family of transcription factors. It consists of 4 members: Fos, FosB, FosL1, and FosL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP1. As such, the Fos proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the Fos gene has also been associated with apoptotic cell death. The phosphorylation of c-Fos at Theorine 232 was critical in regulating cell proliferation and differentiation. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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