Phosphothreonine(pThr)

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Antigen Preparation
A chemically linked phosphothreonine.
Background
"Protein phosphorylation is involved in cell signaling pathways. These cascades are mediated by three types of kinases: serine, threonine and tyrosine kinases which phosphorylate serine, threonine and tyrosine amino acid side chains.These three amino acids are phosphorylated by its specific kinases. These processes are regulated by kinases and phosphatases."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes phosphothreonine (pThr) only. It does not cross react with phosphoserine or phosphotyrosine
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 620-670
Lot #: See the label
Size: 100ug/200ul
Host: Rabbit
Clone:
Isotyping: Rabbit IgG
Applications: IHC(P), WB
Reactivity: all species
Price: $ 245.00
REFERENCES
"Trinidad JC, Thalhammer A, Specht CG, Lynn AJ, Baker PR, Schoepfer R, Burlingame AL. Quantitative analysis of synaptic phosphorylation and protein expressio. Mol. Cell Proteomics 7 (4): 684–96, 2008"
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