Neuropilin-1 (Paired T916)
Neuropilin-1; transmembrane receptor; VEGF165R; NRP1; CD304
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Antigen Preparation
A synthetic peptide surrounding the epitope –LNTQS- without phosphorylation site at Thr916. This sequence is identical among human and bovine.
Background
"The neuropilin-1 (Nrp1) is a multifunctional protein, identified principally as a receptor for the class 3 semaphorins and members of the vascular endothelial growth factor (VEGF) family, but it is capable of other interactions. It is a marker of regulatory T cells (Tr), which often carry Nrp1 and latency-associated peptide (LAP)-TGF-beta1 (the latent form). Nrp1 is reported to be expressed in breast cancer cells. The ischemia can increase neuropilin 1 protein expression in experimental rat brain as well. The phosphorylation of Nrp1 is essential for the Nrp1 activation."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
This antibody recognizes ~130 kDa of human Neuropilin1 protein without phosphorylation site of Thr 916. It does not cross react to phospho Neuropilin 1. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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