MEK5 (MAPKK5) (pS311/pT315)
MAPKK5; Mitogen-activated protein kinase kinase 5
Download Product PDF
MSDS Download Product PDF
Antigen Preparation
"A synthetic peptide surrounding the epitope –SIAKT- with double phosphorylation sites Ser311 and Thr315. This sequence is identical among human, rat, mouse, chicken, and dog."
Background
"The MEK5 (also known as MKK5, MAPKK5, SKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. It catalyzes tyrosine phosphorylation of ERK5 whereas MEK5 itself is regulated by MEKK3. An important link between MEK5 and metastatic prostrate cancer has been demonstrated. Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
|
SPECIFICITY
"This antibody recognizes ~49 kDa of human MEK5 protein at the phosphorylation site of Ser 311/Thr315. It does not cross react to non-phospho MEK5. It also reacts with mouse and rat, chicken. The other species are not tested."
|
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
|
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
|
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|