MEK1/2(pS218/S222)
Mitogen-activated protein kinase kinase 1; MAPKK1; MEK1; MKK1
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Antigen Preparation
"A synthetic peptide surrounding the epitope –SMANS- with double phosphorylation sites Ser218 and Ser222. This sequence is identical among human, rat, mouse, chicken."
Background
"The MEK1 & 2 (MAPKK1/2) are members of tyrosine/threonine protein kinase family that activate the ERK1+2/MAPK enzymes by phosphorylation. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEKs. The MEK1 & 2 are regulated by phosphorylation by one of the MEK kinases."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
"This antibody recognizes ~44/45 kDa of human MEK1 protein at the phosphorylation site of Ser 218/222 or MEK2(pS222/226). It does not cross react to non-phospho MEK1/2. This antibody also reacts with mouse and rat, chicken. The other species are not tested."
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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