STAT1 (pY701)
signal transducer and activator of transcription 1, 91kDa
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Antigen Preparation
"A synthetic peptide surrounding to the epitope -TGYIK-- with phosphorylation sites at Tyr701 of STAT1 protein from human, mouse and rat origins."
Background
"STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT1 at Tyr701 induces STAT1 dimerization, nuclear translocation and DNA binding. STAT 1 is activated by a number of different ligands, including IFN, EGF, PDGF and IL6. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions. "
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes STAT1 (pY701) with a phosphorylated sites of Tyr701. It does not cross-react with non-phosphospecific peptide.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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