EGFR(pS1070)
epidermal growth factor receptor; HER1; Proto-oncogene c-ErbB-1
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Antigen Preparation
A synthetic phosphor-peptide –FLQRY-S-SDPT corresponding to residues surrounding Ser1070 of human EGF receptor.
Background
"EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for
members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding
of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation.
Mutations in this gene are associated with lung cancer. "
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes EGFR with the phosphorylation site Ser1070. It does not cross-react with non-phosphospecific peptide.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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