Antigen Preparation
A synthetic peptide surrounding to the epitope -TPY- with dual phosphorylation sites at Thr183 and Tyr185 of human JNK/SAPK .
Background
"c-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK). There are three isoforms, JNK1 (~49 kDa), JNK2 (~55 kDa), and JNK3 (~54 kDa). JNK is dually phosphorylated at Thr 183 and Tyr 185 by protein kinases MKK4 and MKK7. The JNK and p38 pathways function to modulate cell-cycle, apoptotic and transcriptional responses to stress."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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