Antigen Preparation
A synthetic peptide derived from N-terminus of human BIM.
Background
"Bim (bcl-2-interacting mediator of cell death, BCL-2-related Ovarian Death Gene) is a proapoptotic member of the Bcl-2 family that shares the homology domain3 (BH3) with this family. Bim exists in three isoforms: BimEL, BimL, and BimS. As the BCL-2 family members reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria, they play a pivotal role in deciding whether a cell will live or die. In healthy cells, BIM molecules are localized to the microtubule-associated dynein motor complex through association with the LC8 dynein light chain. Following a death signal, LC8 and BIM dissociate from the motor complex and together translocate to the mitochondria. At the mitochondria, BIM is thought to interact with BCL-2 to antagonize its antiapoptotic activity."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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