Antigen Preparation
A synthetic peptide corresponding to aa 41-54 of human BCL-2 alpha oncoprotein.
Background
"The Bcl-2 gene codes for a 26 kDa protein which has been implicated in the control of cell death by apoptosis, in normal tissues, expression of bcl-2 is restricted to cells and tissues that are characterized by apoptotic cell turnover. Bcl-2 protein exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release, thus it is also considered as an inner mitochondrial membrane protein which can be expressed in lymphoma and breast carcinoma cells. This protein modulates mitochondrial calcium homeostasis and proton flux. BCL-2 protein is frequently overexpressed in many lymphoid malignancies, thus it can immunohistologically distinguish neoplastic germinal centers from reactive ones."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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