Antigen Preparation
A synthetic peptide corresponding to C-terminus of human CD8 protein.
Background
"The CD8 antigen, also known as T8 or Leu2 or Lyt2 or T cell coreceptor, is a cell surface glycoprotein as a
dimer with a molecular mass of 32 kD. It is found on most cytotoxic T lymphocytes that mediates efficient cellcell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the
T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC
molecules. The T8 antigen is expressed by the suppressor/cytotoxic subset of T lymphocytes which comprise
most of the cortical thymocytes and approximately 30% of peripheral blood T cells. Studies have demonstrated
that increased levels of T8+ cells are associated with viral infections such as hepatitis B, Epstein-Barr, and
cytomegalovirus. This antibody may be used in the study of cell-mediated cytotoxicity and that of
immunoregulation and T-lymphocyte-mediated suppression. This antibody stains CD8 (T8) antigen
suppressor/cytotoxic T lymphocytes and majority of thymocytes (approximately 80%) in frozen tissue sections.
"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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