STAT5A (pY694)
Signal transducer and activator of transcription 5

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Product Preparation
A synthetic peptide surrounding to the epitope --KAVDG-Y-VKPQI- with phosphorylation sites at Tyrosine 694 of STAT5a protein from human, mouse, rat, chicken dog and bovine origins.
Background
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. Activation of STAT5a induces dimerization, nuclear translocation and DNA binding. Stat5A is activated by IL-2, IL-7, IL-15, IL-3, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin; activation and dimerization of Stat5A requires phosphorylation of tyrosine 694.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes STAT5a (pY694) with a phosphorylated sites of Ser694. It does not cross-react with non-phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
6776
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-12084
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC, WB, ELISA
Reactivity: Hu, Ms
Price: $ 225.00
REFERENCES
Lin,J.X., Mietz,J., Modi,W.S., John,S. and Leonard,W.J. Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells. J. Biol. Chem. 271 (18), 10738-10744 (1996).
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