STAT3 (pS727)
Signal transducer and activator of transcription 3

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Product Preparation
A synthetic peptide surrounding to the epitope -PMSPR-- with phosphorylation sites at Ser727 of STAT3 protein from human, mouse, rat, chicken dog and bovine origins.
Background
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT3 induces STAT dimerization, nuclear translocation and DNA binding. Stat3 is constitutively activated in a number of human tumors. Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes STAT3 (pS727) with a phosphorylated sites of Ser 727. It does not cross-react with non-phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
6774
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
N/A
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 500-12044
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC, WB, ELISA, IP
Reactivity: Hu, Ms, Rt
Price: $ 240.00
REFERENCES
Ritsuko Matsuo et al. A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody. Journal of Immunological Methods. Volume 247, Issues 1-2, 1 January 2001, Pages 141-151
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