CD15
FUT4; SSEA-1

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Product Preparation
The human myelomonocytic leukemia cells.
Background
CD15 is an antigen present on myelomonocytic cells and Lacto-Nfucopentose III (also known as hapten X). It is present on greater than 90% of granulocytes including neutrophils and eosinophils, and to a lesser degree, on monocytes. CD15 is expressed in Reed-Sternberg cells of Hodgkin’s disease (of the nodular sclerosis, mixed cellularity and lymphocyte-depleted subtypes). This antibody recognizes myeloid cells, mainly granulocytes but not on B cells, T cells, monocytes, erythrocytes or platelets. Positive staining for CD15 combined with a negative reaction for lymphocytic markers may provide support for Hodgkin’s disease. Fifty percent or more of adenocarcinomas have been described as showing significant cytoplasmic positivity for CD15. It does not detect most mesotheliomas.
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
This antibody reacts with human CD15. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
2526
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-1204
Lot #: See the label
Size: 100 ug
Host: Mouse
Isotyping: Mouse IgM
Applications: IHC
Reactivity: Hu
Price: $ 199.00
REFERENCES
Venkataraman,G., Raffeld,M., Pittaluga,S. and Jaffe,E.S. CD15-expressing nodular lymphocyte-predominant Hodgkin lymphoma. Histopathology 58 (5), 803-805 (2011).
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