CK-18 (pS33)
Keratin 18; CK18

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Product Preparation
A synthetic peptide surrounding to the epitope -SAASVYA- with a phosphorylation site at Serine 33 of Cytokeratin 18 protein from human origin. It is identical among human, mouse, rat, etc..
Background
Keratin polypeptides 8 (basic keratin) and 18 (acidic keratin) exist in combination as K8/K18 which are the cytoskeletal intermediate filament proteins of hepatocytes while K8/K18/K19 are the keratins of hepatobiliary ductal cells. Hepatocyte K8/K18 are highly abundant and behave as stress proteins with injury-inducible expression. K8 and K18 are cytoprotective stress proteins that play a central role in guarding hepatocytes from apoptosis. Hepatocellular carcinoma have been reportedly defined by the use of antibodies that recognize only cytokeratins 8 and 18. K18 Ser33 as an interphase phosphorylation site, which increases its phosphorylation during mitosis in cultured cells and regenerating liver, and as an in vitro cdc2 kinase phosphorylation site.Phosphorylation of Ser33 and Ser52 are involved in keratin subcellular distribution.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes CK18 with a phosphorylated site of Ser33. It does not cross-react with non-phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
3875
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 630-110
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB
Reactivity: Hu
Price: $ 345.00
REFERENCES
Ku NO, Liao J, Omary MB, et al. Phosphorylation of human keratin 18 serine 33 regulates binding to 14-3-3 proteins. EMBO J. 1998 Apr 1; 17(7):1892-906.
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