AEG1(C-Term)
Metadherin; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3

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Product Preparation
A synthetic peptide derived from the c-term of human AEG1. This sequence is identical to human, mouse, rat and bovine
Background
AEG1 (Astrocyte elevated gene 1), also called Metadherin (MTDH), or L LYsine-RIch CEACAM1 co-isolated (LYRIC), is 582 aa single transmembrane domain (TMD) protein, this protein is widely expressed and highly conserved between species. It is not a structural component for tight junctional (TJ) complexes, it is recruited during the maturation of the tight junction complex and co-localizes with tight junction proteins ZO-1 and occludin in polarized epithelial cells. This novel protein is also reported as an HIV-1 inducible gene, which is expressed ubiquitously with higher expression in tissues containing muscular actin and its expression is increased in astrocytes infected with HIV-1 or treated with gp120 or tumor necrosis factor (TNF)-alpha. AEG1 expression is elevated in subsets of breast carcinomas, malignant gliomas and melanomas. AEG1 may affect tumor progression in multiple cell lineages by augmenting expression of the transformed phenotype and/or by inducing glutamate excitotoxicity in malignant glioma. In these contexts, an HIV-1-inducible gene, AEG-1, may contribute to multiple brain abnormalities and tumor formation, by both common and distinct mechanisms.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes ~64 kDa of human AEG1 protein. It also reacts with human, mouse and rat origins. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
92140
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 630-100
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB
Reactivity: Hu, Ms, Rt, Bv
Price: $ 245.00
REFERENCES
Sutherland HG, et al. 3D3/lyric: a novel transmembrane protein of the endoplasmic reticulum and nuclear envelope, which is also present in the nucleolus. Exp Cell Res. 2004 Mar 10;294(1):94-105.
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