AEG1(pS568)
Metadherin; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3

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Product Preparation
A synthetic peptide derived from a phosphrylation site of serine 568 surrounding the epitope -TSWESPKQI-of human AEG1. This sequence is identical to human, mouse, rat and bovine.
Background
AEG1 (Astrocyte elevated gene 1), also called MTDH (Metadherin), or L LYsine-RIch CEACAM1 co-isolated (LYRIC), is 582 aa single transmembrane domain (TMD) protein. It is widely expressed and highly conserved between species. It is recruited during the maturation of the tight junction complex and co-localizes with tight junction proteins ZO-1 and occludin in polarized epithelial cells. AEG-1 is involved in several crucial aspects of tumor progression, including transformation, evasion of apoptosis, invasion, metastasis, and chemoresistance. Overexpression of AEG-1 is frequently observed in melanoma, glioma, neuroblastoma, and carcinomas of breast, prostate, liver, and esophagus and is correlated with poor clinical outcomes. AEG-1 functions as a downstream mediator of the transforming activity of oncogenic Ha-Ras and c-Myc. Furthermore, AEG-1 overexpression activates the PI3K/Akt, nuclear factor ?B (NF?B), and Wnt/ß-catenin signaling pathways to stimulate proliferation, invasion, cell survival, and chemoresistance. The lung-homing domain of AEG-1 also mediates the adhesion of tumor cells to the vasculature of distant organs and promotes metastasis. These findings suggest that therapeutic targeting of AEG-1 may simultaneously suppress tumor growth, block metastasis, and enhance the efficacy of
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes phosphospecific AEG1at phosphorylated Ser568. It does not recognize non-phosphspecific site Ser568. This antibody reacts with human, mouse and rat origins. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
92140
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 630-080
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB
Reactivity: Hu, Ms, Rt, Bv
Price: $ 345.00
REFERENCES
Sutherland HG, et al. 3D3/lyric: a novel transmembrane protein of the endoplasmic reticulum and nuclear envelope, which is also present in the nucleolus. Exp Cell Res. 2004 Mar 10;294(1):94-105.
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