IKK-a (pS176/180)
I-kappa-B kinase-1

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Product Preparation
A synthetic peptide surrounding of –QGSLCTS-of human IKK-a/ß protein with a single phosphorylation site. This sequence is identical to human, rat, mouse and bovine.
Background
Nuclear factor kappa B (NFkappaB) is a ubiquitous and an essential mediator of gene expression during activation of immune and inflammatory responses. NFkappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NFkappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NFkappaB activity. IkappaB is phosphorylated by IkappaB kinase (IKK) complex that contains IKKalpha, IKKbeta, and IKKgamma. A novel molecule in the IKK complex was recently identified and designated IKKepsilon/IKK-iota. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes phosphorylated Serine 176 of human IKK-alpha/beta protein. It does not recognize the nonphosphorylated IKK-a/ß.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
1147
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 620-820
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB, IHC
Reactivity: hu, Ms, Rt,
Price: $ 345.00
REFERENCES
Delhase,M., et al. Positive and negative regulation of IkappaB kinase activity through IKKbeta subunit phosphorylation. Science 284 (5412), 309-313 (1999)
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