Neuropilin-1 (Paired T916)
Neuropilin-1; transmembrane receptor; VEGF165R; NR

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Product Preparation
A synthetic peptide surrounding the epitope –LNTQS- without phosphorylation site at Thr916. This sequence is identical among human and bovine.
Background
The neuropilin-1 (Nrp1) is a multifunctional protein, identified principally as a receptor for the class 3 semaphorins and members of the vascular endothelial growth factor (VEGF) family, but it is capable of other interactions. It is a marker of regulatory T cells (Tr), which often carry Nrp1 and latency-associated peptide (LAP)-TGF-beta1 (the latent form). Nrp1 is reported to be expressed in breast cancer cells. The ischemia can increase neuropilin 1 protein expression in experimental rat brain as well. The phosphorylation of Nrp1 is essential for the Nrp1 activation.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes ~130 kDa of human Neuropilin1 protein without phosphorylation site of Thr 916. It does not cross react to phospho Neuropilin 1. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
8829
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 620-340
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Wb, ELISA, IP, IHC
Reactivity: Hu, Bv
Price: $ 325.00
REFERENCES
·         Eldar Hochman et al. Molecular Pathways Regulating Pro-migratory Effects of Hedgehog Signaling. J. Biol. Chem., Vol. 281, Issue 45, 33860-33870, November 10, 2006
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