Neuropilin-1 (pT916)
Neuropilin-1; transmembrane receptor; VEGF165R; NR

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Product Preparation
A synthetic peptide surrounding the epitope –LNTQS- with a phosphorylation site at Thr916. This sequence is identical among human and bovine.
Background
The neuropilin-1 (Nrp1) is a multifunctional protein, identified principally as a receptor for the class 3 semaphorins and members of the vascular endothelial growth factor (VEGF) family, but it is capable of other interactions. It is a marker of regulatory T cells (Tr), which often carry Nrp1 and latency-associated peptide (LAP)-TGF-beta1 (the latent form). Nrp1 is reported to be expressed in breast cancer cells. The ischemia can increase neuropilin 1 protein expression in experimental rat brain as well. The phosphorylation of Nrp1 is essential for the Nrp1 activation.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~130 kDa of human Neuropilin1 protein at the phosphorylation site of Thr 916. It does not cross react to non-phospho Neuropilin 1. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
8829
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 620-330
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Wb, ELISA, IP, IHC
Reactivity: Hu, Bv
Price: $ 375.00
REFERENCES
·         Eldar Hochman et al. Molecular Pathways Regulating Pro-migratory Effects of Hedgehog Signaling. J. Biol. Chem., Vol. 281, Issue 45, 33860-33870, November 10, 2006
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