MAPKK5 (MEK5) (pS311/pT315)
MAPKK5; Mitogen-activated protein kinase kinase 5

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Product Preparation
A synthetic peptide surrounding the epitope –SIAKT- with double phosphorylation sites Ser311 and Thr315. This sequence is identical among human, rat, mouse, chicken, and dog.
Background
The MEK5 (also known as MKK5, MAPKK5, SKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. It catalyzes tyrosine phosphorylation of ERK5 whereas MEK5 itself is regulated by MEKK3. An important link between MEK5 and metastatic prostrate cancer has been demonstrated. Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~49 kDa of human MEK5 protein at the phosphorylation site of Ser 311/Thr315. It does not cross react to non-phospho MEK5. It also reacts with mouse and rat, chicken. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
5607
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 620-300
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Wb, ELISA, IP
Reactivity: Hu, Ms, Rt
Price: $ 375.00
REFERENCES
Tetsuro Shishido, et al. Effects of MEK5/ERK5 Association on Small Ubiquitin-Related Modification of ERK5: Implications for Diabetic Ventricular Dysfunction After Myocardial Infarction. Circulation Research. 2008; 102:1416
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