Neurofibromin(pS2741)
NF1 neurofibromin 1

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Product Preparation
A synthetic peptide surrounding the epitope –PTSPY- with a phosphorylation site of Ser2741 of human neurofibromin. This sequence is identical among human, rat, mouse, chicken, bovine and dog.
Background
The neurofibromatosis type 1 (NF1) tumor suppressor (neurofibromin) is thought to play crucial roles in cellular Ras- and cAMP-dependent kinase (PKA)-associated signals. The absence of or alteration of the neurofibromin protein may lead to neurofibromatosis disease. Recently, a cellular neurofibromin-associating protein, NG,NG-dimethylarginine dimethylaminohydrolase (DDAH)has been identified as a cellular NO/NOS regulator which increases PKA phosphorylation of native neurofibromin in a dose-dependent manner. The PKA accessibility of neurofibromin regulated via DDAH interaction may modulate the cellular function of neurofibromin that is implicated in NF1-related pathogenesis.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~240 kDa of human neurofibromin protein at the phosphorylation site of Ser 2741. It does not cross react to non-phospho NF1.This antibody also reacts with mouse, rat, chicken, bovine and dog. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
4763
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 620-240
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Wb, ELISA, IP
Reactivity: Hu
Price: $ 290.00
REFERENCES
Tokuo H. Phosphorylation of neurofibromin by cAMP-dependent protein kinase is regulated via a cellular association of NG,NG-dimethylarginine dimethylaminohydrolase .  FEBS Letters , Volume 494 , Issue 1 - 2 , Pages 48 - 53
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