MEK1/2(pS218/S222)
Mitogen-activated protein kinase kinase 1; MAPKK1;

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Product Preparation
A synthetic peptide surrounding the epitope –SMANS- with double phosphorylation sites Ser218 and Ser222. This sequence is identical among human, rat, mouse, chicken.
Background
The MEK1 & 2 (MAPKK1/2) are members of tyrosine/threonine protein kinase family that activate the ERK1+2/MAPK enzymes by phosphorylation. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEKs. The MEK1 & 2 are regulated by phosphorylation by one of the MEK kinases.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~44/45 kDa of human MEK1 protein at the phosphorylation site of Ser 218/222 or MEK2(pS222/226). It does not cross react to non-phospho MEK1/2. This antibody also reacts with mouse and rat, chicken. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
415549
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 620-190
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Wb, ELISA, IP
Reactivity: Hu, Ms, Rt
Price: $ 290.00
REFERENCES
Ali R. Jazirehi, et al. Inhibition of the Raf–MEK1/2–ERK1/2 Signaling Pathway, Bcl-xL Down-Regulation, and Chemosensitization of Non-Hodgkin’s Lymphoma B Cells by Rituximab. CANCER RESEARCH 64, 7117–7126, October 1, 2004
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