STAT1 (pY701)
signal transducer and activator of transcription 1

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Product Preparation
A synthetic peptide surrounding to the epitope -TGYIK-- with phosphorylation sites at Tyr701 of STAT1 protein from human, mouse and rat origins.
Background
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT1 at Tyr701 induces STAT1 dimerization, nuclear translocation and DNA binding. STAT 1 is activated by a number of different ligands, including IFN, EGF, PDGF and IL6. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes STAT1 (pY701) with a phosphorylated sites of Tyr701. It does not cross-react with non-phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
6772
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 620-160
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 250.00
REFERENCES
Tong R Wu, et al. SHP-2 Is a Dual-specificity Phosphatase Involved in Stat1 Dephosphorylation at Both Tyrosine and Serine Residues in Nuclei, J. Biol. Chem., Vol. 277, Issue 49, 47572-47580, December 6, 2002
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