Antigen Preparation
Mice were immunized with culture human peripheral blood monocytes.
Background
"CD14, also known lipopolysaccharide (LPS) receptor, is a 55 kDa glycosylphosphatidylinositol (GPI)-linked membrane
glycoprotein. It is expressed at high levels on monocytes and macrophage, and at lower levels on the surface of
granulocytes and neutrophils. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells,
and Langerhans cells have also been reported to express CD14. CD14 is anchored to cells by linkage to GPI and functions
as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at
physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell
activation. CD14 is involved in the clearance of gram-negative pathogens and in the up-regulation of adhesion molecules
and cytokines expression in monocytes and neutrophils"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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