Abbomax

Career

CD3(T3)-Hu

CD3

Antigen Preparation

Human T cells expressed CD3

Background

CD3 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. This anti-CD3 antibody is commonly used as a phenotypic marker for human T cells.

PURIFICATION The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping	SPECIFICITY This antibody recognizes human CD3 protein. The other species was not tested.
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.	STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

919

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

5-10 µg/ml

Order Info

Catalog #:	603-030
Lot #:	See the label
Size:	100ug
Host:	Mouse
Clone:	UCHT-1
Isotyping:	IgG1
Applications:	ELISA, WB, IHC, FC
Reactivity:	Hu
Price:	$ 250.00

REFERENCES

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfidelinked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):141527.